SANS SANS-SEC504 : Hacker Tools, Techniques, Exploits and test Dumps

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Exam Number : SANS-SEC504
Exam Name : Hacker Tools, Techniques, Exploits and Incident Handling
Vendor Name : SANS
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SANS-SEC504 test Format | SANS-SEC504 Course Contents | SANS-SEC504 Course Outline | SANS-SEC504 test Syllabus | SANS-SEC504 test Objectives

- How to best prepare for an eventual breach
- The step-by-step approach used by many computer attackers
- Proactive and reactive defenses for each stage of a computer attack
- How to identify active attacks and compromises
- The latest computer attack vectors and how you can stop them
- How to properly contain attacks
- How to ensure that attackers do not return
- How to recover from computer attacks and restore systems for business
- How to understand and use hacking tools and techniques
- Strategies and tools for detecting each type of attack
- Attacks and defenses for Windows, UNIX, switches, routers, and other systems
- Application-level vulnerabilities, attacks, and defenses
- How to develop an incident handling process and prepare a team for battle
- Legal issues in incident handling

- Preparation
- Building an incident response kit
- Identifying your core incident response team
- Instrumentation of the site and system
- Identification
- Signs of an incident
- First steps
- Chain of custody
- Detecting and reacting to insider threats
- Containment
- Documentation strategies: video and audio
- Containment and quarantine
- Pull the network cable, switch and site
- Identifying and isolating the trust model
- Eradication
- Evaluating whether a backup is compromised
- Total rebuild of the Operating System
- Moving to a new architecture
- Recovery
- Who makes the determination to return to production=>
- Monitoring to system
- Expect an increase in attacks
- Special Actions for Responding to Different Types of Incidents
- Espionage
- Inappropriate use
- Incident Record-keeping
- Pre-built forms
- Legal acceptability
- Incident Follow-up
- Lessons learned meeting
- Changes in process for the future
- Reconnaissance
- What does your network reveal=>
- Are you leaking too much information=>
- Using forward and reverse Whois lookups, ARIN, RIPE, and APNIC
- Domain Name System harvesting
- Data gathering from job postings, websites, and government databases
- Recon-ing
- Pushpin
- Identifying publicly compromised accounts
- Maltego
- FOCA for metadata analysis
- Aggregate OSINT data collection with SpiderFoot
- Scanning
- Locating and attacking personal and enterprise Wi-Fi
- Identifying and exploiting proprietary wireless systems
- Rubber Duckie attacks to steal Wi-Fi profiles
- War dialing with War-VOX for renegade modems and unsecure phones
- Port scanning: Traditional, stealth, and blind scanning
- Active and passive operating system fingerprinting
- Determining firewall filtering rules
- Vulnerability scanning using Nessus and other tools
- Distributing scanning using cloud agents for blacklist evasion
- Intrusion Detection System (IDS) Evasion
- Foiling IDS at the network level
- Foiling IDS at the application level: Exploiting the rich syntax of computer languages
- Web Attack IDS evasion tactics
- Bypassing IDS/IPS with TCP obfuscation techniques
- Enumerating Windows Active Directory Targets
- Windows Active Directory domain enumeration with BloodHound, SharpView
- Windows Command and Control with PowerShell Empire
- Operating system bridging from Linux to Windows targets
- Defending against SMB attacks with sophisticated Windows networking features
- Physical-layer Attacks
- Clandestine exploitation of exposed USB ports
- Simple network impersonation for credential recovery
- Hijacking password libraries with cold boot recovery tool
- Gathering and Parsing Packets
- Active sniffing: ARP cache poisoning and DNS injection
- Bettercap
- Responder
- LLMNR poisoning
- WPAD attacks
- DNS cache poisoning: Redirecting traffic on the Internet
- Using and abusing Netcat, including backdoors and insidious relays
- IP address spoofing variations
- Encryption dodging and downgrade attacks
- Operating System and Application-level Attacks
- Buffer overflows in-depth
- The Metasploit exploitation framework
- AV and application whitelisting bypass techniques
- Netcat: The Attacker's Best Friend
- Transferring files, creating backdoors, and shoveling shell
- Netcat relays to obscure the source of an attack
- Replay attacks
- Endpoint Security Bypass
- How attackers use creative office document macro attacks
- Detection bypass with Veil, Magic Unicorn
- Putting PowerShell to work as an attack tool
- AV evasion with Ghostwriting
- Attack tool transfiguration with native binaries
- Password Cracking
- Password cracking with John the Ripper
- Hashcat mask attacks
- Modern Windows Pass-the-Hash attacks
- Rainbow Tables
- Password guessing and spraying attacks
- Web Application Attacks
- Account harvesting
- SQL Injection: Manipulating back-end databases
- Session cloning: Grabbing other users' web sessions
- Cross-site scripting
- Denial-of-Service Attacks
- Distributed Denial of Service: Pulsing zombies and reflected attacks
- Local Denial of Service
- Maintaining Access
- Backdoors: Using Poison Ivy, VNC, Ghost RAT, and other popular beasts
- Trojan horse backdoors: A nasty combo
- Rootkits: Substituting binary executables with nasty variations
- Kernel-level Rootkits: Attacking the heart of the Operating System (Rooty, Avatar, and Alureon)
- Covering the Tracks
- File and directory camouflage and hiding
- Log file editing on Windows and Unix
- Accounting entry editing: UTMP, WTMP, shell histories, etc.
- Covert channels over HTTP, ICMP, TCP, and other protocols
- Sniffing backdoors and how they can really mess up your investigations unless you are aware of them
- Steganography: Hiding data in images, music, binaries, or any other file type
- Memory analysis of an attack
- Putting It All Together
- Specific scenarios showing how attackers use a variety of tools together
- Analyzing scenarios based on real-world attacks
- Learning from the mistakes of other organizations
- Where to go for the latest attack info and trends
- Hands-on Analysis
- Nmap port scanner
- Nessus vulnerability scanner
- Network mapping
- Netcat: File transfer, backdoors, and relays
- Microsoft Windows network enumeration and attack
- More Metasploit
- Exploitation using built in OS commands
- Privilege escalation
- Advanced pivoting techniques
- How to best prepare for an eventual breach
- The step-by-step approach used by many computer attackers
- Proactive and reactive defenses for each stage of a computer attack
- How to identify active attacks and compromises
- The latest computer attack vectors and how you can stop them
- How to properly contain attacks
- How to ensure that attackers do not return
- How to recover from computer attacks and restore systems for business
- How to understand and use hacking tools and techniques
- Strategies and tools for detecting each type of attack
- Attacks and defenses for Windows, UNIX, switches, routers, and other systems
- Application-level vulnerabilities, attacks, and defenses
- How to develop an incident handling process and prepare a team for battle
- Legal issues in incident handling

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getting ready your Submission | SANS-SEC504 test Cram and cheat sheet

This area will support you when getting ready your manuscript for preliminary submission and resubmission to Nature Protocols. For tips on our goals & scope, as well as our content material forms, please check with the concerning the journal part. upon getting organized your manuscript, the the way to post part will provide you with information on the submission gadget, whereas our editorial standards and approaches are described in the Peer evaluate and book area.

On this web page: Editorial and publishing policies |  Protocol format  |  figure format  |  Nomenclature  |  existence sciences reporting instructions  |  Copyright  |  ebook permissions  |  Self-archiving

Editorial and publishing guidelines

Nature Protocols is a discussion board for the book of confirmed Protocols. hence, we do not publish novel primary analysis and Protocol authors ought to have used their method to generate results posted in a peer-reviewed primary research journal. nonetheless, we admire that Protocols are invariably evolving, so if the methodology has better for the reason that the long-established ebook, authors may still notify us of the changes and provide us with the latest edition of the protocol. The procedure may additionally deviate a bit of from the version used in the aiding paper, however any important advancements, diversifications, or extensions of a Protocol may still be supported by way of the ebook of an extra supporting basic research paper. 

As a part of Nature research, Nature Protocols follows a number of ordinary guidelines (as specific on our editorial policies web page) and we request that authors abide by them. In specific, should you publish a manuscript to Nature Protocols, its content material need to no longer greatly overlap with some other papers from you or your co-authors’ businesses which are beneath consideration or in press at other journals. if you post a linked manuscript to any other journal whereas the submission to Nature Protocols is under consideration, you must send us a copy of the related manuscript and details of its development against ebook. We reserve the right to say no booklet of a protocol even after it has been permitted if it turns into obvious that there are severe issues with the scientific content material or violations of our publishing policies.

in addition to the normal Nature analysis policies, we provide additional Nature Protocols-certain assistance on averting talents replica ebook and self-plagiarism. We require that each one Nature Protocols articles are in response to strategies used in as a minimum one posted basic analysis paper. despite the fact, they should at all times extend upon the suggestions obtainable in the assisting paper(s), enabling clients to extra conveniently put into effect the approach of their own lab. extra, Nature Protocols articles ought to comprise tips not already available somewhere else within the scientific literature. to satisfy this requirement, a Nature Protocols article often must be focused on a way for which the authors haven't currently posted a detailed protocol. If a method has in the past been published in a protocol layout, it is commonly most effective acceptable to publish a Nature Protocols article after the publication of at the least one further basic research paper that demonstrates extra building of the approach (e.g. refinement, optimization or extended applications). We therefore ask that knowledge authors upload, together with their Nature Protocols manuscript, a copy of:

  • at the least one helping basic paper from the writer’s lab where the method has been used.
  • another publication(s) focused on the method with one or greater authors in regular with the proposed Nature Protocols article.
  • proposing finished tips about old publications early within the editorial procedure helps to make certain the nature Protocols article augments the posted literature and avoids talents problems with self-plagiarism and reproduction e-book. Submission is taken to indicate that all co-authors have approved the contents of the protocol and its submission with the aid of the corresponding creator, and that the corresponding creator is approved to signify all co-authors in pre-book discussions with the journal. The corresponding creator for editorial applications doesn't deserve to be the senior writer or the adult to whom correspondence is addressed after booklet. The fundamental affiliation for each and every author may still be the institution the place the vast majority of their work became finished. If an writer has consequently moved, the existing handle may also be brought up.

    Protocol format

    We suggest that every one protocol authors follow these guidelines anyplace viable to facilitate the refereeing and editorial procedure. Microsoft word is our favored layout for text; youngsters, we are able to also settle for simple ASCII textual content (.txt) and RTF layout (.rtf). in the event you are able to submit, please determine your manuscript the usage of our writer guidelines.

    Title: The title should succinctly describe the formula and, if acceptable, its software. it's going to be no longer than 30 phrases.

    Authors: Please include people who have contributed intellectually and just about to the building of the method. For all authors, please include first identify, core initial (if acceptable), last names, postal tackle (department, institution, metropolis and nation) and e-mail address. additionally consist of the telephone number and fax quantity for the corresponding author. A hyperlink to a lab domestic web page or team of workers information web page may also even be covered for acceptable authors.

    abstract: Please consist of a abstract of the protocol (maximum 250 phrases), in short describing the protocol and its applications. The suggested structure is to consist of one or two sentences summarizing the protocol, then explain the levels of the protocol and how these examine with other protocols, after which consist of one or two sentences describing the results that can also be anticipated. If viable, consist of a ultimate sentence indicating how lengthy the whole protocol takes and no matter if a particular stage of talents is required. The abstract should still include information in regards to the method itself in preference to a abstract of the effects which have been acquired in the past the usage of the formula.

    Introduction: The purpose of the introduction is to enable readers to make a choice as to the suitability of the protocol to their experimental problem. initially make sure to introduce the method beneath discussion. encompass references to key papers the place the protocol has been used in the past, together with these published via your own community, and any stories that talk about functions of the protocol. you're actively inspired, the place acceptable, to reference different protocols in Nature Protocols. Use subheadings the place applicable; these might encompass:

  • construction of the protocol: consist of references to your personal peer-reviewed basic analysis publications.
  • functions of the formula: in case you feel the protocol can be adapted for use in a much wider range of functions than offered, you'll want to focus on the whole range of the purposes of the method.
  • comparison with different strategies: the place relevant, reference should be made to option strategies which are common to obtain the same/similar results as the protocol. The benefits and disadvantages of your protocol compared to other options may still be discussed.
  • Experimental design: in this area, please provide all tips in regards to the design of the experiments that readers would should be in a position to adapt the protocol to their personal experimental situation. This section should still also include a discussion of the controls integral for the protocol. For protocols describing the education of biological molecules, a scheme displaying the sequence of reactions should be included (all schemes may still be labeled as figures).
  • skills essential to implement the protocol: If any a part of the protocol cannot be implemented by means of a reliable graduate student or postdoc, or if implementation requires the involvement of a specialised core facility (e.g. animal husbandry, deep sequencing), please recommend who can be most certified to function it. This additionally applies where any a part of the protocol may still be carried out by using a couple of person concurrently.
  • limitations: The possible boundaries of the protocol (including any cases where the protocol is probably going to be unreliable or unsuccessful) should be discussed.
  • materials: Please include a listing of the essential substances, under headings REAGENTS and gadget. This may still encompass tips in regards to the suppliers used for reagents (e.g., company, web address and catalog number). when you have discovered that deviations from a specific reagent, or its source, have antagonistic outcomes on the effect of the protocol this should be made clear by means of the word crucial adopted by using a short clarification. poisonous or hazardous agents should be made clear by the note warning adopted through a quick clarification of the hazard and the precautions that may still be taken when coping with the agent. Please additionally spotlight any professional gadget required. Please deliver as lots suggestions as which you can to support users efficiently determine the product, e.g. company identify, company web site, product code. in case you need to encompass distinct suggestions about specific reagents or device, please list in extra non-compulsory sections, called REAGENT SETUP and device SETUP. These sections are relevant for particulars of composition of buffers or the setup of equipment. within these sections, the title of the reagent or machine kinds a 2d degree of subheading.

    REAGENT SETUP is the appropriate part to consist of particulars involving, as an instance: the mandatory pattern specification (when it comes to minimal protein quantity and allowed buffer add-ons) for a mass spectrometry experiment; particulars for getting ready a sophisticated buffer; and the pre-treatment of solvents or reagents to make certain they're moisture-free or air-free. For every merchandise, please point out whether it will be made up fresh or may also be saved (if so, beneath which situations and for a way long). moreover, please state whether % options are wt/vol or vol/vol. equipment SETUP is the acceptable area to encompass particulars involving, for instance, the setup of HPLC separation strategies. consist of diagrams or photographs of equipment set-up, the place appropriate.

    For protocols that use reside vertebrates or greater invertebrates, authors ought to state that each one experiments should still be carried out in line with vital instructions and laws. For manuscripts reporting experiments on human subjects, authors should additionally consist of a press release confirming that counseled consent have to be received from all topics. These statements should still appear as CAUTIONS. These have to be positioned within the reagents section, technique and within the legend of any tables and figures that exhibit records accrued using human or animal subjects. 

    process: This have to be offered as a numbered record of direct experimental directions. Please use the lively demanding as opposed to the passive demanding. If the protocol naturally breaks into separate tiers, then encompass subheadings and resume the numbered list. include a TIMING callout with each subheading and state how lengthy the section will take to comprehensive. Subheadings are specifically acceptable after steps where the process can also be stopped and resumed at a later date, which should still be indicated with a PAUSE element flag adopted with the aid of a quick description of the alternate options accessible (e.g. "can also be left overnight at 4°C or frozen for as much as a month at -20°C").

    spotlight critical steps within the protocol that must be performed in a extremely precise method to maximise the likelihood of success (e.g. where the time and temperature of a step is critical or the use of RNase free options is required). Make these clear with a critical STEP flag adopted by way of a brief explanation. highlight any toxic or harmful chemical substances with a warning flag upon their first mention and encompass quick details of the hazard and the acceptable coping with suggestions. Please state all centrifugation speeds in g and include the length of time and temperature of the centrifugation (e.g. centrifuge at 14,000 g for 5 mins, four°C). Please encompass TROUBLESHOOTING flags after steps where issues are encountered. include full particulars of the problem and options in the Troubleshooting section.

    where there are alternative routes to attain the subsequent stage of the protocol, please deliver sufficient advice for readers to make an recommended resolution on the path to select. Letters of the Latin alphabet (A), B), C)...) may still be used to determine the different alternatives, and Roman numerals (i), ii), iii)...) may still be used to damage down the applicable steps. if you want to encompass subheadings, add the subheading initially of the first step within that area and make the text italic. This formatting can even be used to encompass second-degree subheadings in the leading process. If the protocol is complex, agree with including a move diagram to demonstrate how the degrees healthy together. We motivate authors to submit movies for above all advanced steps or stages.

    Timing: If viable, please encompass a timeline indicating the approximate time each and every step or stage will take (e.g. Steps 1–three, 30 min; Steps 4+5, 2 h). Please additionally deliver this assistance as an inventory at the conclusion of the system. if you feel it would be more person friendly, which you can display timing for every area or day of the protocol instead.

    Troubleshooting: Please encompass assistance on how to troubleshoot the absolutely issues users will stumble upon with the protocol. Please supply this advice within the kind of a table with the column headings ‘step’, ‘difficulty’, ‘feasible cause’, and ‘solution’. The step quantity should still be given for the place the issue is first followed. The acceptable steps should even be highlighted within the technique with TROUBLESHOOTING flags. If troubleshooting text refers to just one or two steps, it may possibly also be formatted as regular textual content with subheadings relating to the critical steps or sections.

    predicted effects: Please consist of counsel about the doubtless influence of protocol (as an example, likely yield of protein, ordinary microscopy pictures, etc.). If feasible, please consist of one set of records from an experiment that worked very neatly and a 2nd for an experiment that required troubleshooting to gain significant outcomes. Please also consist of suggestions on the way to interpret and analyze uncooked statistics, including equations if integral. Analytical statistics for chemical substances synthesized as part of the procedure may still be supplied in following format:

    Fmoc-L-Serine-OAll (1). White strong, mp eighty two.5-84.0 °C

    [α]D = + 0.three (c 7.5 in EtOAc at 20 °C)TLC (CH2Cl2:CH3OH 15:1 v/v) Rf = 0.141H NMR (500 MHz, CDCl) δ 2.33 (br s, 1H), 3.94 (br d, J = 10.3 Hz, 1H), four.03 (br d, J = 10.1 Hz, 1H), four.23 (t, J = 6.9 Hz, 1H), 4.forty three (m, 2H), four.forty eight (m, 1H), four.69 (d, J = 5.three Hz, 2H), 5.26 (dd, J = 0.eight Hz, J = 10.5 Hz, 1H), 5.35 (dd, J = 0.8 Hz, J = 17.2 Hz, 1H), 5.seventy nine (d, J = 7.four Hz, 1H), 5.ninety one (ddt, J = sixteen.6 Hz, J = eleven.0 Hz, J = 5.5 Hz, 1H), 7.32 (br t, J = 7.four Hz, 2H), 7.41 (br t, J = 7.4 Hz, 2H), 7.61 (m, 2H), 7.77 (d, J = 7.6 Hz, 2H)13C NMR (one hundred twenty five MHz, CDCl3) δ 47.30 (CH), fifty six.28 (CH), sixty three.48 (CH2), 66.fifty nine (CH2), 67.42 (CH2), 119.25 (CH2), 120.21 (CH), one hundred twenty.23 (CH), one hundred twenty five.30 (CH), 127.29 (CH), 127.32 (CH), 127.ninety seven (CH), 131.fifty one (CH), 141.fifty one (C), 141.fifty five (C), 143.87 (C), one hundred forty four.02 (C), 156.forty six (C), 170.forty five (C)IR (CH2Cl2, cm-1) 3425, 3064, 2952, 2892, 1725, 1513, 1198FAB-LRMS (m/z)(relative intensity) 368 ([M+1]+, 28 %)analysis (calculated, found for C21H21O5N) C (sixty eight.sixty five, sixty eight.38) H (5.seventy six, 5.59) N (3.eighty one, three.ninety) (Taken from: 10.1038/nprot.2006.470)

    Figures: Please read the digital photos integrity and requirements coverage earlier than making ready your figures. When feasible, we choose to use customary digital figures to be certain the highest quality replica in the journal. All figures should be uploaded separately by the use of our on-line submission device, in one of our favourite formats (see part on figure format under). On preliminary submission, all panels of a figure or table should still be combined into one file; please do not send as separate files. Please encompass a short title and legend for each electronic determine submitted, and a title for each desk. Please make sure that each axes of all graphs are labeled correctly. at the same time as Nature Protocols is a web product, many clients will print your protocol previous to use. as a result figures may still be sized to be legible to users and to facilitate printing. Figures should now not comprise a couple of panel until the materials are logically related; each and every panel of a multipart determine should be sized so that the entire figure will also be reduced by using the same amount and reproduced on the broadcast web page at the smallest measurement at which essential details are seen. When a protocol is accredited for ebook, we could ask for top-decision determine files, might be in a different digital layout. This tips might be included in the acceptance letter.

    Lettering on figures should be in a transparent, sans-serif typeface (as an instance, Helvetica); if feasible, the same typeface and approximate font measurement (no smaller than 7 point) should still be used for all figures in a paper. Use symbol font for Greek letters. Figures may still be on a white heritage, and may evade extreme boxing, unnecessary colour, spurious ornamental outcomes (such as 3D histograms) and totally pixelated computer drawings. The vertical axis of histograms should no longer be truncated to magnify small alterations. Labeling must be of ample size and distinction to be readable after applicable size discount. The thinnest strains in the last determine should still be no smaller than 0.5 factor wide. Authors will see a proof of figures. within your budget requests to enlarge figures should be regarded, however editors will make the ultimate resolution on figure dimension.

    Figures divided into parts should be labeled with lower-case, daring letters (a, b, c...) in the equal typesize as used in other places in the figure. Lettering in figures should still be in lower-case class, with most effective the first letter of each and every label capitalized. contraptions should have a single house between the quantity and the unit, and comply with SI nomenclature (for instance, ms in place of msec) or the nomenclature standard to a specific box. lots should still be separated by means of commas (1,000). odd gadgets or abbreviations should still be spelled out in full or defined in the legend. Scale bars may still be used instead of magnification elements, with the length of the bar defined in the legend as opposed to on the bar itself. In widely wide-spread, please use visual cues as opposed to verbal explanations, akin to "open pink triangles", within the legend.

    We intention to provide chemical structures in a consistent structure all through our articles. Please use the nature research Chemical structures ebook and ChemDraw template to make sure that you put together your figures in a format with the intention to require minimal adjustments by our artwork and creation groups. post final files at 100% as .cdx information.

    Tables: Please put up tables in observe structure at the end of your textual content doc, or as a separate file. Tables should be created the use of the Microsoft word desk editor, the place feasible.

    Supplementary information: Supplementary information should still healthy into one in every of three categories:

    1. extended records: extended information is an essential component of the paper and most effective information that at once contribute to the leading message should still be introduced. These figures could be integrated into the complete-textual content HTML edition of your paper and may be appended to the online PDF. there's a restrict of 10 extended records figures, and each need to be observed generally text/on-line methods ( noted as ‘prolonged records determine 1’, ‘prolonged records determine 2,’ and many others.). every extended facts determine may still be of the same first-rate because the leading figures, and should be offered at a size in an effort to enable both the figure and legend to be offered on a single A4 page. each figure should be submitted as an individual .jpg, .tif or .eps file with a highest measurement of 10 MB every, the use of the ‘figure File’ article category in our Manuscript tracking gadget.

    2. SUPPLEMENTARY suggestions: Supplementary information is material that is elementary background to the examine however which it is not functional to include within the PDF version of the paper (as an example, video data, colossal data sets and calculations). each merchandise have to be talked about more often than not manuscript/on-line methods and distinct in the connected stock of Supplementary tips. Tables containing significant facts units may still be in Excel layout, with the table quantity and title blanketed within the physique of the table. All textual guidance and any additional Supplementary Figures (which may still be offered with the legends at once under every determine) should be offered as a single, mixed PDF. Please be aware that Supplementary suggestions is not copyedited and we cannot exchange any Supplementary information after the paper has been formally approved except there was a critical scientific error. Supplementary Figures and other items are not required to be referred to as out on your manuscript textual content, however should be numerically numbered, starting at one, as ‘Supplementary figure 1’, etc., now not SI1.

    3. supply statistics: We motivate you to supply supply records to your Figures each time feasible. Full-length, unprocessed blots and gels must be provided as supply data for any primary Figures, and will be provided as individual PDF information (one for every determine) containing all aiding blots and/or gels with the linked figure noted within the PDF file. records underlying any critical graphical Figures should be provided as individual Excel files (one for each and every determine) with the linked figure stated in the Excel file. We encourage deposition of all types of supply statistics right into a principal repository, for instance figshare ( or the graphic facts useful resource ( Please see extra counsel about facts availability:

    every piece of SI may still be mentioned so as in the textual content. Please checklist all items of Supplementary advice, including a title and legend for each, at the end of your Manuscript File. If any references are mentioned in SI that are not included generally reference list, please include full citation particulars within the legend for the SI (for items with legends) or in a reference checklist within the individual SI merchandise.  If any references are stated in each the leading text and the SI, they may also be repeated in the SI reference record. Please use one among our permitted titles on your SI:

  • Supplementary Audio(s) (numbered)
  • Supplementary Audio Legend(s) (optional, to accompany audios; posted in on-line Supp. data. title page rather than as separate file)
  • Supplementary records (numbered if >1)
  • Supplementary discussion
  • Supplementary Equation(s) (one file; use plural "Equations" in title if a couple of equation is protected in file)
  • Supplementary figure(s) (at all times numbered)
  • Supplementary determine Legend(s) (required, as a part of determine or accompanying determine)
  • Supplementary manual
  • Supplementary application
  • Supplementary methods
  • Supplementary word(s) (numbered if >1)
  • Supplementary results
  • Supplementary Sequence Archive
  • Supplementary table(s) (all the time numbered)
  • Supplementary Tutorial
  • Supplementary Video(s) (always numbered)
  • Supplementary Video Legend(s) (not obligatory, to accompany video clips; posted in on-line Supp. data. title page in place of as separate file)
  • File sizes should still be as small as viable (optimum size of 30 MB) in order that they can be downloaded at once. The mixed complete measurement of all info have to not exceed one hundred fifty MB. Supplementary Figures need to be supplied in JPEG layout in RGB coloration mode, and at a resolution of between a hundred and fifty-300 dpi. Allowable formats for different forms of SI consist of (however aren't limited to) right here: Adobe Acrobat file (.pdf), Audio visual Interleave (.avi), Compressed Archive file (.zip), Flash film (.swf), HTML doc (.html), MPEG animation (.mpg), MS Excel spreadsheet (.xls, .xlsx), simple ASCII text (.txt), QuickTime movie (.mov), rich text layout (.rtf), methods Biology Markup Language (.sbml, .xml, .owl), TAR archive file (.tar), and Waveform audio file (.wav). If a file structure that you simply want to consist of as Supplementary counsel is not listed, please contact our editorial crew, who will recommend you as to whether its inclusion is viable.

    creator contributions statements: Authors are required to encompass a statement of responsibility within the manuscript that specifies the contribution of each author. Examples of published statements can also be viewed at this Nautilus submit ( Nature journals additionally enable two coauthors to be special as having contributed equally to the work being described (most often used for co-first authors), however decide on using specified author contributions statements.

    ORCID for corresponding authors: As a part of our efforts to Boost transparency in authorship, we request that all corresponding authors of published papers supply their Open Researcher and Contributor Identifier (ORCID) identification, earlier than resubmitting the final version of the manuscript. ORCID helps the scientific neighborhood obtain unambiguous attribution of all scholarly contributions.

    Corresponding authors should hyperlink their ORCID to their account in the manuscript monitoring equipment (MTS). From the MTS homepage, click modify my Springer Nature account after which ORCID Create/hyperlink an Open Researcher Contributor identification (ORCID) in the own Profile tab. this may re-direct you to the ORCID web site. if you have already got an ORCID account, enter your ORCID e-mail and password and click on on Authorize. in case you don’t have one, that you would be able to create one at this stage. Linking ORCID and MTS money owed may also be accomplished at any time just before acceptance. For extra suggestions please discuss with ORCID at Springer Nature. if you journey technical considerations please contact the Platform assist Helpdesk.

    Non-corresponding authors would not have to link their ORCID but are inspired to accomplish that. Please notice that it isn't possible so as to add/adjust ORCID details at proof.

    Acknowledgments: Please word an acknowledgments area can be covered.

    Competing pursuits: Submission of a signed Competing pursuits commentary is required for all content of the journal. This observation can be posted at the end of all papers, even if or no longer a competing pastime is said. In circumstances the place the authors declare a competing hobby, a short remark to that effect is posted at the end of article, which is linked to a more special edition purchasable online.

    References: References are numbered sequentially as they seem within the text, determine legends, tables and containers. Use superscript numbers to point out a reference, for example 1. just one book is given for each quantity, and footnotes aren't used. in your numbered checklist, consist of handiest papers which have been posted through a named ebook or which have been permitted to a identified preprint server. meeting abstracts and papers in training should be outlined in the textual content with a listing of authors (or initials if any of the authors are co-authors of the existing contribution). Patents should still be blanketed in the reference list. published conference abstracts and URLs for web websites should be referred to parenthetically in the text, not within the reference record. furnish details and acknowledgments don't seem to be permitted as numbered references.

    All authors may still be covered in reference lists except there are greater than five, wherein case most effective the primary author may still accept, followed through 'et al.'. Authors should still be listed remaining name first, adopted through a comma and initials of given names. Titles of mentioned articles are required and may be in Roman textual content and titles of books in italics; the first notice of the title is capitalized, the title written exactly because it appears within the work mentioned, ending with a full stop. Journal names are italicized and abbreviated (with full stops) in keeping with commonplace usage; consult with the country wide Library of medicine for particulars. quantity numbers seem in daring. For booklet citations, the writer and city of ebook are required (e.g. John Wiley & Sons, Hoboken, New Jersey, united states of america, 2003). Please see examples under:

  • Helms, C. et al. A putative RUNX1 binding site variant between SLC9A3R1 and RAT9 is linked to susceptibility to psoriasis. Nat. Genet. 35, 349-356 (2003).
  • Lovett, M. Direct choice of cDNAs with huge genomic DNA clones. In Molecular Cloning: A Laboratory guide Edn. 3 Vol. 2 (eds. Sambrook., J. & Russell, D.W.) eleven.98-eleven.133 (bloodless Spring Harbor Laboratory Press, bloodless Spring Harbor, ny, country, 2001).
  • Petroff, M.D. & Stapelbroek, M.G. Blocked impurity band detectors. US Patent 4,586,960 filed 23 Oct. 1980, and issued 4 Feb. 1986.
  • Babichev, S. A., Ries, J. & Lvovsky, A. I. Quantum scissors: teleportation of single-mode optical states through skill of a nonlocal single photon. Preprint at (2002).
  • determine structure

    making ready production first-class figures: Please study the digital photos integrity and requirements coverage. When feasible, we favor to use normal digital figures to make sure the highest-great copy within the journal. For most efficient results, prepare figures at real size for the journal. Figures that don't meet these necessities will no longer reproduce well and booklet may well be delayed unless we acquire excessive-resolution images. We cannot offer to deliver corrected reprints with greater image excellent if handiest bad first-rate photographs have been offered at accept stage. Authors are liable for informing the journal of any necessities to attain permission to publish any figures or illustrations that are protected by using copyright, including figures posted elsewhere and pictures taken by using professional photographers. The journal cannot publish photographs downloaded from the web without acceptable permission.

    When developing and submitting digital information, please comply with the instructions discovered here. See web page three of the doc for information selected to Nature Protocols. 

    photo forms: photos fall into two simple classes: photographic or scanned photographs, and graphs and schematic diagrams. The most efficient structure for any specific determine relies upon partly on what variety of pictures it consists of. Rasterized codecs are most advantageous for images, scans, and composite figures containing photographic or scanned images with minimal or relatively essential labeling, whereas line or vector codecs are optimum for graphs, schematic diagrams, and composite figures containing a combination of photos or scans and exact textual content or line artwork.

    digital photograph formats: a large choice of software is obtainable to generate and manipulate photographs, and a big range of snap shots file codecs exist. Rasterized (or bitmapped) codecs such as TIFF are composed of an array of dots (pixels). The satisfactory is determined by the resolution, usually measured in dots per inch (dpi; often known as 'pixels per inch'). For satisfactory copy, we want at the very least 300 dpi at the measurement the photograph is to seem. measurement and resolution are linked such that enlarging a picture to twice its fashioned dimension will halve the resolution. If the decision is just too low, particular person pixels come into view to the attention, the perimeters of lines start to seem 'stepped' and the graphic can also seem blurred or pixelated. Line (or vector) codecs akin to Postscript, EPS and PDF hold particular person lines and textual content as separate, editable accessories. This makes them less demanding and sooner to reletter or edit as imperative, reducing the chance of error, and provides sharper consequences in print and on-line. additionally, as a result of these pictures have no 'resolution' as such, they may well be enlarged with none discount in high-quality. leave all layers intact in TIFF and EPS info. Our favourite codecs are:

  • Adobe Photoshop (layered .psd file best) or TIFF layout (excessive decision, minimum 300 dpi) for photographic photos. Please ship the Photoshop file with the layers intact. individual accessories of the determine cannot be edited on flattened Photoshop data. colossal TIFF data can be compressed (LZW compression is favourite), which does not degrade the great of the image. then again, compression application similar to DropStuff or ZipIt will also be used to in the reduction of the file sizes.
  • Adobe Illustrator, Postscript, EPS or PDF format for figures containing line drawings and graphs, including figures combining text and line paintings with photos or scans. If these formats don't seem to be viable, we will also settle for Microsoft note, Microsoft Excel, Microsoft PowerPoint or JPEG (high-resolution, 300 dpi, as separate information (no longer embedded in your textual content file). youngsters PowerPoint can export JPEGs, the resulting files are low resolution and not proper for printing. The simplest way to attain an effective photographs structure from Microsoft workplace functions is to generate Postscript using 'Print to file'.
  • QuickTime film (.mov) or Flash film (.swf) structure for movies, or audio information (.wav). films should still be lower than 10MB in dimension; however in case your movie is greater than 10MB, please let us know and we will do our most suitable to accommodate it.
  • Nomenclature

    Authors may still use accepted nomenclature for gene symbols. the complete identify should still be provided originally mention, the gene image in brackets i.e. titin(Ttn). Thereafter, the gene symbol should be used. Please talk to the appropriate nomenclature databases for correct gene names, symbols and formatting. A effective resource is Entrez Gene. accredited human gene symbols are provided by HUGO Gene Nomenclature Committee (HGNC), e mail: authorized mouse symbols are offered by means of The Jackson Laboratory, e mail: a further valuable web page is the Gene Ontology project. steer clear of record assorted names of genes (or proteins) separated by means of a shrink, as in 'Oct4/Pou5f1', as here's ambiguous (it may suggest a ratio, a posh, choice names or distinctive subunits). Use one name all the way through and include the different originally point out: 'Pou5f1 (often known as Oct4)'.


    Authors of manuscripts authorised for booklet might be sent a copyright transfer form, please contact us if you want additional advice at this stage.

    ebook permissions

    if your tables, figures, photos or videos have been in the past posted, please cite their original vicinity of ebook within the legend and tell us so we attain e-book permission. Citations of non-public communications should be approved through the correspondent concerned, with signed permission despatched to the editorial workplace. it is courteous to inform the author of your intent to use their published work. If we're unable to gain the indispensable rights for some images to be re-used or tailored in our articles, we will contact you to focus on the sourcing of choice cloth.

    Self archiving

    Authors are in a position to self-archive the creator edition of their authorized manuscript (notice or textual content files produced with the aid of the writer, now not a PDF or HTML downloaded from six months after ebook. The article will were peer reviewed, but it would not have been edited into the final published edition. This policy has been developed to meet the needs of authors and the evolving policies of funding businesses that might also want to archive the research they fund. it's also designed to offer protection to the integrity of the scientific listing, with the published edition naturally identified because the definitive version of the article.

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